Item – Thèses Canada

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Numéro d'OCLC
63124690
Lien(s) vers le texte intégral
Exemplaire de BAC
Exemplaire de BAC
Auteur
Boivin, Éric,1980-
Titre
Développement d'un système rapporteur visuel permettant de mesurer la fréquence des changements de cadre de lecture chez Arabidopsis thaliana.
Diplôme
Thèse (M. Sc.)--Université Laval, 2004.
Éditeur
Ottawa : Library and Archives Canada = Bibliothèque et Archives Canada, [2005]
Description
1 microfiche
Notes
Comprend des références bibliographiques
Résumé
In this study, a reporter allowing us to measure the frequency of frameshift mutations in plants was developed. The reporter construct was introduced into the genome of 'Arabidopsis thaliana' via ' Agrobacterium'-mediated transformation. Five single-copy lines were obtained and three of these showed a low, but constant mutation frequency over the T3, T4 and T5 generations. A UV treatment applied to two reporter lines resulted in a significant increase (~18 fold) in the mutation rate in both lines. This response to a DNA damaging agent validated the potential of these lines as tools to measure the frequency of frameshift mutations in plants. The insertion sites of the T-DNA in the reporter lines were finally determined since the lines will eventually be used by crossing them with mutants deficient in different DNA repair mechanisms.
ISBN
0612980634
9780612980631